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Igor K Lednev |
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Associate Professor Analytical Chemistry Department of Chemistry
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Congrats Dr. Vitali!Congratulations on Vitali Sikirzhytski who has successfully passed his PhD defense!
Recent publicationsStructural Variations in the Cross-β Core of Amyloid β Fibrils Revealed by Deep UV Resonance Raman Spectroscopy Kelly Virkler and Igor K. Lednev - Anal. Chem., 2009, 81 (18), pp 7773–7777
Species in a snap: Raman analysis of blood - Research Profile Erika Gebel - Anal. Chem., 2009, 81 (19), p 7862 “For forensic purposes, Raman is great,” says Igor Lednev, who is at the University at Albany, State University of New York, and coauthored the paper with his student Kelly Virkler. ... In the study, Lednev and Virkler collected Raman spectra from 24 dry blood samples: 8 each from humans, cats, and dogs... |
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Understanding fibrillogenesis at a molecular level requires detailed structural characterization of amyloid fibrils. The combination of deep UV resonance Raman (DUVRR) spectroscopy and post mortem hydrogen−deuterium exchange (HX) was utilized for probing parallel vs antiparallel β-sheets in fibrils prepared from full-length Aβ1−40 and Aβ34−42 peptides, respectively. Using previously published structural data based on solid-state NMR analysis, we verified the applicability of Asher’s approach for the quantitative characterization of peptide conformation in the Aβ1−40 fibril core. We found that the conformation of the parallel β-sheet in the Aβ1−40 fibril core is atypical for globular proteins, while in contrast, the antiparallel β-sheet in Aβ32−42 fibrils is a common structure in globular proteins. In contrast to the case for globular proteins, the conformations of parallel and antiparallel β-sheets in Aβ fibril cores are substantially different, and their differences can be distinguished by DUVRR spectroscopy.
Forensic analysis has become one of the most growing areas of analytical chemistry in recent years. The ability to determine the species of origin of a body fluid sample is a very important and crucial part of a forensic investigation. We introduce here a new technique which utilizes a modern analytical method based on the combination of Raman spectroscopy and advanced statistics to analyze the composition of blood traces from different species. Near-infrared Raman spectroscopy (NIR) was used to analyze multiple dry samples of human, canine, and feline blood for the ultimate application to forensic species identification. Of the six principle components that were determined to be present, the first three, which contributed over 90% to the spectral data of the system, were used to form a three-dimensional scores plot that clearly showed significant separation between the three groups of species. Ellipsoids representing a 99% confidence interval surrounding each species group showed no overlap. This technique using Raman spectroscopy is nondestructive and quick and can potentially be performed at the scene of a crime.